Data for the Constructed Mutant: YOJLd

General Information

Name of the MutantYOJLd (disruption)
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1242 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YOJLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagc tag) yojL-F: aagaagcTTGTAGCCGGCTTGGCTG
Reverse Primer:(ggaggatc tag) yojL-R: ggaggatcCTTTGTCCGACATAAAGC
Length of the Cloned Region: 14 to 200 Length: 187
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YOJLd