Data for the Constructed Mutant: YFNFd

General Information

Name of the MutantYFNFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size909 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFNFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) nFHF: gccgaagcttAGATTATATCAGGCAGTCG
Reverse Primer:(gcgcggatcc tag) nFBR: gcgcggatccATTGCTGTTCAGCACAGC
Length of the Cloned Region: 37 to 285 Length: 249
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFNFd