Data for the Constructed Mutant: YFNEd

General Information

Name of the MutantYFNEd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1176 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFNEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) nEHF: gccgaagcttTCCGCTGATCTTTACACG
Reverse Primer:(gcgcggatcc tag) nEBR: gcgcggatccGCTTTGCGAGCTGTTCC
Length of the Cloned Region: 168 to 421 Length: 254
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFNEd