Data for the Constructed Mutant: YFMSd

General Information

Name of the MutantYFMSd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size858 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFMSd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) mSHF: gccgaagcttCCGCAGATATTCTGGACG
Reverse Primer:(gcgcggatcc tag) mSBR: gcgcggatccAATTCTTTCGGAACCATGC
Length of the Cloned Region: 32 to 257 Length: 226
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFMSd