Data for the Constructed Mutant: YFMKd

General Information

Name of the MutantYFMKd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size444 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFMKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) mKHF: gccgaagcttTGCTGCTATTAGATGAGTG
Reverse Primer:(gcgcggatcc tag) mKBR: gcgcggatccTCAGGTTCGGTCATCAGC
Length of the Cloned Region: 101 to 305 Length: 205
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFMKd