Data for the Constructed Mutant: YFMKd
General Information
Name of the Mutant
YFMKd (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
HindIII and BamHI
Gene Size
444 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YFMKd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
gccgaagctt
tag)
mKHF:
gccgaagcttTGCTGCTATTAGATGAGTG
Reverse Primer:
(
gcgcggatcc
tag)
mKBR:
gcgcggatccTCAGGTTCGGTCATCAGC
Length of the Cloned Region: 101 to 305 Length: 205
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YFMKd