Data for the Constructed Mutant: YFMAdd

General Information

Name of the MutantYFMAdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhoI-BamHI & HindIII-XhoI
Gene Size165 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YFMAdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gccgctcgag tag) yfmA-UF:gccgctcgagTGCTTTCTCATGAAAGCATC
Reverse Primer:(cgcggatc tag) yfmA-UBR:cgcggatcCACGACCATGAAACGCTG
Downstream:
Forward Primer:(gcgaagc tag) yfmA-DHF:gcgaagcTTTCAGGTACAGACGATCG
Reverse Primer:(gccgctcgag tag) yfmA-DR: gccgctcgagTCGTTATCTGTCACAAAGAG
Linearization:
Length of the Deletion: 9 to 143 Length: 135


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFMAdd