Data for the Constructed Mutant: YFLMd

General Information

Name of the MutantYFLMd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1008 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFLMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) LM-1H: gccgaagcttACATACGAAGGAAGAGC
Reverse Primer:(cgcggatcc tag) LM-2B: cgcggatccGTCTGACTCATATCCAG
Length of the Cloned Region: 57 to 336 Length: 280
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFLMd