Data for the Constructed Mutant: YFLLdd

General Information

Name of the MutantYFLLdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhoI-BamHI & HindIII-XhoI
Gene Size273 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFLLdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gccgctcgag tag) yflL-UF:gccgctcgagTCATCAAGTCTGAACACATC
Reverse Primer:(cgcggatc tag) yflL-UBR:cgcggatcCAATGATTCGGTATTGAAGC
Downstream:
Forward Primer:(cgcaagctt tag) yflL-DHF:cgcaagcttCATCTCTGTGACTGAATCC
Reverse Primer:(gccgct tag) yflL-DR: gccgctCGAGAAACCTCGCAGATG
Linearization:
Length of the Deletion: 23 to 212 Length: 190


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFLLdd