Data for the Constructed Mutant: YFLCdd

General Information

Name of the MutantYFLCdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhoI-BamHI & EcoRI-XhoI
Gene Size150 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFLCdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gccgctcg tag) yflC-UF:gccgctcgAGAGTCCAAAGCCAAGAAAG
Reverse Primer:(cgcggatcc tag) yflC-UBR:cgcggatccAAAAATCCATCATTTCACCTC
Downstream:
Forward Primer:(cgcgaatt tag) yflC-DEF:cgcgaattCTTGCCGGAATCGCTGC
Reverse Primer:(gccgctcg tag) yflC-DR: gccgctcgAGATATATGCTCTATCCATAC
Linearization:
Length of the Deletion: 13 to 116 Length: 104


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFLCdd