Data for the Constructed Mutant: YFLAd

General Information

Name of the MutantYFLAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1413 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFLAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) lAHF: gccgaagcttTATATCTGATTGGCTGTGG
Reverse Primer:(gcgcggatcc tag) lABR: gcgcggatccAGGCAATAAGCCACATCC
Length of the Cloned Region: 30 to 316 Length: 287
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFLAd