Data for the Constructed Mutant: YFKSdd

General Information

Name of the MutantYFKSdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhopI-BamHI & HindIII-XhoI
Gene Size198 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFKSdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcg tag) yfkS-UF:ctcctcgAGACCTGCCGAATCAAATG
Reverse Primer:(ggaggatc tag) yfkS-UBR:ggaggatcCTGTACGATATAGCTGATC
Downstream:
Forward Primer:(aagaagc tag) yfkS-DHF:aagaagcTTTATATGAGAGTGAATCCC
Reverse Primer:(ctcctcg tag) yfkS-DR: ctcctcgAGAACAGCCATTGGTGTTC
Linearization:
Length of the Deletion: 22 to 136 Length: 115


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFKSdd