Data for the Constructed Mutant: YFKOd

General Information

Name of the MutantYFKOd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size663 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFKOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgaagctt tag) kOHF: gcgaagcttCGTGAATACACATG
Reverse Primer:(gccggatcc tag) kOBR: gccggatccTGTCACTTTCAAGCAAATGC
Length of the Cloned Region: 196 to 409 Length: 214
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFKOd