Data for the Constructed Mutant: YFKKdd

General Information

Name of the MutantYFKKdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhoI-BamHI & HindIII-XhoI
Gene Size213 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFKKdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gccgctcga tag) yfkK-UF:gccgctcgaGCTGATTCCTTCTCTGCG
Reverse Primer:(cgcggatcc tag) yfkK-UBR:cgcggatccGGACAAAGGGTTCCATCC
Downstream:
Forward Primer:(gcgaagctt tag) yfkK-DHF:gcgaagcttAGTGAAATGCAGGCGATTG
Reverse Primer:(gccgctcgag tag) yfkK-DR: gccgctcgagCGGTGATAAAGGAATACCG
Linearization:
Length of the Deletion: 7 to 168 Length: 162


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFKKdd