Data for the Constructed Mutant: YFKAd

General Information

Name of the MutantYFKAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size462 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YFKAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) kAHF: gccgaagcttAAGCTGCGTCCGATTACG
Reverse Primer:(gcgcggatcc tag) kABR: gcgcggatccATGCTGATGGATCTGAGG
Length of the Cloned Region: 16 to 248 Length: 233
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFKAd