Data for the Constructed Mutant: YFJRd

General Information

Name of the MutantYFJRd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size783 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:

Mutant Construction for YFJRd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jRHF: gccgaagcttGACAGAAGACCTTGTCAC
Reverse Primer:(gcgcggatcc tag) jRBR: gcgcggatccCCTTTCTCTGCATGAGCC
Length of the Cloned Region: 30 to 260 Length: 231
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFJRd