Data for the Constructed Mutant: YFJQd

General Information

Name of the MutantYFJQd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size957 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFJQd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jQHF: gccgaagcttTCAACATTACCGCAATCAC
Reverse Primer:(gcgcggatcc tag) jQBR: gcgcggatccTTTGGCCGCTGCATATGC
Length of the Cloned Region: 5 to 200 Length: 196
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFJQd