Data for the Constructed Mutant: YFJOd

General Information

Name of the MutantYFJOd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1398 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFJOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jOHF: gccgaagcttGATTCTCATGATATCGTGC
Reverse Primer:(gcgcggatcc tag) jOBR: gcgcggatccAGCTTGACGGTCTGTTCC
Length of the Cloned Region: 484 to 914 Length: 431
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFJOd