Data for the Constructed Mutant: YFJMd

General Information

Name of the MutantYFJMd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size450 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFJMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jMHF: gccgaagcttAACGGCTATGCATCATCC
Reverse Primer:(gcgcggatcc tag) jMBR: gcgcggatccTCGTAATTTCCCGAAACGA
Length of the Cloned Region: 5 to 209 Length: 205
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFJMd