Data for the Constructed Mutant: YFJEd

General Information

Name of the MutantYFJEd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size456 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFJEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jEHF: gccgaagcttCGTTTGAATCTAAATATGCC
Reverse Primer:(gcgcggatcc tag) jEBR: gcgcggatccTGGCGTCCCATATAAAGC
Length of the Cloned Region: 17 to 224 Length: 208
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFJEd