Data for the Constructed Mutant: YFJDd

General Information

Name of the MutantYFJDd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFJDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jDHF: gccgaagcttATGTTCCTGAGAATATGGG
Reverse Primer:(gcgcggatcc tag) jDBR: gcgcggatccGTCTTGATGCGGAAAATGG
Length of the Cloned Region: 37 to 245 Length: 209
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFJDd