Data for the Constructed Mutant: YFJBd

General Information

Name of the MutantYFJBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1221 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFJBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) jBHF: gccgaagcttAAACATTGCAGTTGATCCG
Reverse Primer:(gcgcggatcc tag) jBBR: gcgcggatccTCACTCAGCTTAGTGACG
Length of the Cloned Region: 9 to 212 Length: 204
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFJBd