Data for the Constructed Mutant: YFIZd

General Information

Name of the MutantYFIZd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size999 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFIZd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iZHF: gccgaagcttTTTACTGACGGCTGTCTG
Reverse Primer:(gcgcggatcc tag) iZBR: gcgcggatccAGCGCCTGCATGAGAGC
Length of the Cloned Region: 57 to 251 Length: 195
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIZd