Data for the Constructed Mutant: YFIXd

General Information

Name of the MutantYFIXd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1818 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFIXd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iXHF: gccgaagcttCTTCGGCTCGTTTGACC
Reverse Primer:(gcgcggatcc tag) iXBR: gcgcggatccACGTTTGTCGTCTCAATTG
Length of the Cloned Region: 12 to 227 Length: 216
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIXd