Data for the Constructed Mutant: YFIVd

General Information

Name of the MutantYFIVd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size480 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YFIVd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iVHF: gccgaagcttATTTACACGATACAACAGTC
Reverse Primer:(gcgcggatcc tag) iVBR: gcgcggatccGCAATGTCGCTCACTTTC
Length of the Cloned Region: 11 to 209 Length: 199
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFIVd