Data for the Constructed Mutant: YFISd

General Information

Name of the MutantYFISd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1251 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFISd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iSHF: gccgaagcttCGCAATCAAAAAGGACTG
Reverse Primer:(gcgcggatcc tag) iSBR: gcgcggatccTCAGATAGAAACATGATCGT
Length of the Cloned Region: 19 to 248 Length: 230
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFISd