Data for the Constructed Mutant: YFIRd

General Information

Name of the MutantYFIRd (disruption)
Used VectorpMutin2
Used Enzyme SitesEcoRI and BamHI
Gene Size615 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:

Mutant Construction for YFIRd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccggaattc tag) iREF: gccggaattcACAAGGATAAACGGCAGG
Reverse Primer:(gcgcggatcc tag) iRBR: gcgcggatccTCAAGCTTTCGGAGTCCT
Length of the Cloned Region: 26 to 227 Length: 202
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIRd