Data for the Constructed Mutant: YFIRd
General Information
Name of the Mutant
YFIRd (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
EcoRI and BamHI
Gene Size
615 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
Correspondence:
Mutant Construction for YFIRd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
gccggaattc
tag)
iREF:
gccggaattcACAAGGATAAACGGCAGG
Reverse Primer:
(
gcgcggatcc
tag)
iRBR:
gcgcggatccTCAAGCTTTCGGAGTCCT
Length of the Cloned Region: 26 to 227 Length: 202
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YFIRd