Data for the Constructed Mutant: YFHPd

General Information

Name of the MutantYFHPd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size981 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFHPd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) HYFHP: gccgaagcttAAAAACAATGCTGTT
Reverse Primer:(gcgggatcc tag) BYFHP: gcgggatccTACCAAATCGCGATAGCTG
Length of the Cloned Region: 157 to 455 Length: 299
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFHPd