Data for the Constructed Mutant: YFHKd

General Information

Name of the MutantYFHKd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size516 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFHKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) hKHF: gccgaagcttAGAAACAAGTAATGCTCGC
Reverse Primer:(gcgcggatcc tag) hKBR: gcgcggatccTGTTCTGATGATACAGTGC
Length of the Cloned Region: 8 to 215 Length: 208
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFHKd