Data for the Constructed Mutant: YFHEdd

General Information

Name of the MutantYFHEdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhoI-BamHI & EcoRI-XhoI
Gene Size108 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFHEdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcga tag) yfhE-UF:ctcctcgaGCGGAACGATCGGAACG
Reverse Primer:(ggagga tag) yfhE-UBR:ggaggaTCCATAAAAAGGACCTCC
Downstream:
Forward Primer:(gcggaattc tag) yfhE-DEF:gcggaattcAAAGCTGCTAAGCGGAAG
Reverse Primer:(ctcctcga tag) yfhE-DR: ctcctcgaGGATAATGAGCGTTTCGC
Linearization:
Length of the Deletion: 6 to 90 Length: 85


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFHEdd