Data for the Constructed Mutant: YFHDdd

General Information

Name of the MutantYFHDdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesXhoI-BamHI & HindIII-XhoI
Gene Size189 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFHDdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcgag tag) yfhD-UF:ctcctcgagATTGCACGGGTGAAGCAC
Reverse Primer:(ggaggatcc tag) yfhD-UBR:ggaggatccATATGATTTCTGCCCATATC
Downstream:
Forward Primer:(aagaagctt tag) yfhD-DHF:aagaagcttAGCGCAAGAGCGTGCG
Reverse Primer:(ctcctcgag tag) yfhD-DR: ctcctcgagTTCAAAAACACACTTGGGC
Linearization:
Length of the Deletion: 18 to 137 Length: 120


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFHDdd