Data for the Constructed Mutant: YDJLd

General Information

Name of the MutantYDJLd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1038 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDJLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) : gccgaagcttAAATGGTGCGGCATCTG
Reverse Primer:(cgcggatcc tag) : cgcggatccTTTGTCGCCTGCTTTGA
Length of the Cloned Region: 94 to 513 Length: 420
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDJLd