Data for the Constructed Mutant: YDIRd

General Information

Name of the MutantYDIRd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size939 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YDIRd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) : gccgaagcttGCCGGAGTATCTAGTAC
Reverse Primer:(cgcggatcc tag) : cgcggatccATCTTCCTCAATATCAA
Length of the Cloned Region: 58 to 597 Length: 540
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDIRd