Data for the Constructed Mutant: YCZIdd

General Information

Name of the MutantYCZIdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size243 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YCZIdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcgag tag) yczI-F-F(Xho):ctcctcgagGGTGCGGAAACGATCTC
Reverse Primer:(ggaggatcc tag) yczI-F-R(Bam):ggaggatccGAAACAAAAGGACCATCC
Downstream:
Forward Primer:(aagaagctt tag) yczI-B-F(Hin) :aagaagcttGGTCTGTCTCCCTTATCGG
Reverse Primer:(ctcctcgag tag) yczI-B-R(Xho) : ctcctcgagCGATCCTCTGGTGAATCTG
Linearization:
Length of the Deletion: 7 to 206 Length: 200


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCZIdd