Data for the Constructed Mutant: YCZHD

General Information

Name of the MutantYCZHD (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YCZHD (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yczH1: gcgcaagcttCTTGTCATTCTCGCACACGA
Reverse Primer:(gccgagatct tag) yczH2 : gccgagatctGTAATAACAAACCGATCCGC
Length of the Cloned Region: 16 to 333 Length: 318
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCZHD