Data for the Constructed Mutant: YCZGd

General Information

Name of the MutantYCZGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size312 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCZGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yczG-1: gcgcaagcttCAGAAACAGAAACATTGCAG
Reverse Primer:(gccgagatct tag) yczG-2: gccgagatctCTGCACAGGTGCTGCACGTT
Length of the Cloned Region: 20 to 136 Length: 117
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCZGd