Data for the Constructed Mutant: YCZFdd

General Information

Name of the MutantYCZFdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size219 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YCZFdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcgag tag) yczF-F-F(Xho):ctcctcgagCCCCGCCCAGCGGGAGCG
Reverse Primer:(ggaggatcc tag) yczF-F-R(Bam):ggaggatccGGTCACGCCCAATATC
Downstream:
Forward Primer:(aagaagctt tag) yczF-B-F(Hin):aagaagcttCCCCTAAAGGGGGGCGC
Reverse Primer:(ctcctcgag tag) yczF-B-R(Xho): ctcctcgagTCGTTGACGGTGTGAACG
Linearization:
Length of the Deletion: 22 to 215 Length: 194


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCZFdd