Data for the Constructed Mutant: YCZAdd

General Information

Name of the MutantYCZAdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size159 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YCZAdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcgag tag) yczA-F-F(Xho):ctcctcgagCTGCCCGTTTGCTTGAATAC
Reverse Primer:(ggaggatcc tag) yczA-F-R(Bam) :ggaggatccGACCATTTGATATCCTCC
Downstream:
Forward Primer:(aagaagctt tag) yczA-B-F(Hin):aagaagcttGCATTTGAATAAGTAAAGGAG
Reverse Primer:(ctcctcgag tag) yczA-B-R(Xho): ctcctcgagTTTGATGGACGAGATGCC
Linearization:
Length of the Deletion: 6 to 147 Length: 142


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCZAdd