Data for the Constructed Mutant: YCEJd

General Information

Name of the MutantYCEJd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1170 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCEJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yceJ-1: gcgcaagcttCATGTTTGTCATTGGCACGG
Reverse Primer:(gccgagatgt tag) yceJ-2: gccgagatgtGGACAATGACTGGGATGGAC
Length of the Cloned Region: 30 to 358 Length: 329
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCEJd