Data for the Constructed Mutant: YCEId

General Information

Name of the MutantYCEId (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1200 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCEId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yceI-1: gcgcaagcttTATCCCAGCGTAAACTGCTG
Reverse Primer:(gccgagatct tag) yceI-2: gccgagatctCCCATACCAATCACGAAACG
Length of the Cloned Region: 20 to 335 Length: 316
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCEId