Data for the Constructed Mutant: YCEEd

General Information

Name of the MutantYCEEd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size576 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YCEEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yceE-1: gcgcaagcttGGACAGCGCATTGATTTAAC
Reverse Primer:(gccgagatct tag) yceE-2: gccgagatctCGTGAATGGTCACTGTGATG
Length of the Cloned Region: 22 to 340 Length: 319
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCEEd