Data for the Constructed Mutant: YCEBd

General Information

Name of the MutantYCEBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size939 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCEBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yceB-1: gcgcaagcttAGCATTCGGTTGAGCTTGCC
Reverse Primer:(gccgagatct tag) yceB-2: gccgagatctGTCCGTTAATGCAAGTCTGG
Length of the Cloned Region: 14 to 312 Length: 299
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCEBd