Data for the Constructed Mutant: YCEAd

General Information

Name of the MutantYCEAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size840 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCEAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yceA-1: gcgcaagcttGTCATGGATCCGATACTAGG
Reverse Primer:(gccgagatct tag) yceA-2: gccgagatctTCACAAGCGATCCAAATAGG
Length of the Cloned Region: -840 to -840 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCEAd