Data for the Constructed Mutant: YCDId

General Information

Name of the MutantYCDId (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size693 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCDId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycdI-1: gcgcaagcttTTTCGGCTATTCCCATACGC
Reverse Primer:(gccgagatct tag) ycdI-2: gccgagatctCGATTTTACGATGGCGGAGG
Length of the Cloned Region: 30 to 406 Length: 377
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCDId