Data for the Constructed Mutant: YCDDd
General Information
Name of the Mutant
YCDDd (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
HindIII and BglII
Gene Size
501 (nt)
Growth & Expression (reporter lacZ):
in DSM:
no
in MM:
no
Mutant Submission to NIG:
Correspondence:
Mutant Construction for YCDDd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
gcgcaagctt
tag)
ycdD-1:
gcgcaagcttTCGGCATGAATGGCATTCTC
Reverse Primer:
(
gccgagatct
tag)
ycdD-2:
gccgagatctTCAATGCCTTTATTCGCTGC
Length of the Cloned Region: 72 to 191 Length: 120
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YCDDd