Data for the Constructed Mutant: YCDCd

General Information

Name of the MutantYCDCd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1359 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCDCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycdC-1: gcgcaagcttAGGAGATCGGAAATGTACCG
Reverse Primer:(gccgagatct tag) : gccgagatctCTGAATTGGGAATCGGAAGC
Length of the Cloned Region: 32 to 385 Length: 354
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCDCd