Data for the Constructed Mutant: YCDBd

General Information

Name of the MutantYCDBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1416 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCDBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycdB-1: gcgcaagcttTTGTGAAGAAAGAGGGACTG
Reverse Primer:(gccgagatct tag) ycdB-2: gccgagatctCTCACTTTATCGCCGTATGC
Length of the Cloned Region: 17 to 350 Length: 334
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCDBd