Data for the Constructed Mutant: YCDAd

General Information

Name of the MutantYCDAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1062 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCDAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycdA-1: gcgcaagcttCTGCTGATGATGTTTACAGC
Reverse Primer:(gccgagatct tag) ycdA-2: gccgagatctTTCGCTATAGTCTTCAGGGT
Length of the Cloned Region: 40 to 327 Length: 288
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCDAd