Data for the Constructed Mutant: YCCFd

General Information

Name of the MutantYCCFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1074 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCCFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yccF-1: gcgcaagcttTGAAACGAAATGCCCCTTGC
Reverse Primer:(gccgagatct tag) yccF-2: gccgagatctTTCGGACGAGTGATCGAATC
Length of the Cloned Region: 11 to 341 Length: 331
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCCFd