Data for the Constructed Mutant: YCCCd

General Information

Name of the MutantYCCCd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1125 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCCCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yccC-1: gcgcaagcttGCTCGTACTTTTTACCGCAC
Reverse Primer:(gccgagatct tag) yccC-2: gccgagatctTGCTGCCGACGTTAACAATC
Length of the Cloned Region: 18 to 325 Length: 308
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCCCd