Data for the Constructed Mutant: YBYBdd

General Information

Name of the MutantYBYBdd (deletion (double-cross))
Host168
Used VectorpMutin1
Used Enzyme SitesHindIII;,BamHI;,XhoI
Gene Size258 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YBYBdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gtctcgag tag) ybyB-X1:gtctcgagCTCCGTGGAATCCGACAAAC
Reverse Primer:(gtggatcc tag) ybyB-BA:gtggatccCAGTTGAAACAGCGAGACCTG
Downstream:
Forward Primer:(gtaagctt tag) ybyB-HD:gtaagcttCAGAAGGCTCCATGTATCC
Reverse Primer:(gtctcgag tag) ybyB-X2: gtctcgagCATGGTCGACGCGTTCGG
Linearization:
Length of the Deletion: 44 to 211 Length: 168


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YBYBdd